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KMID : 0903520060490040315
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2006 Volume.49 No. 4 p.315 ~ p.321
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Acetylcholinesterase-based Biosensor for Detection of Residual Organophosphates and Carbamates Insecticides
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Kim Young-Mee
Kim Jin-Young
Cho Moon-Jae
Chang Kong-Man
Hyun Hae-Nam
Kim So-Mi
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Abstract
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Inhibitors of acetylcholinesterase (AchE), such as organophosphates and carbamates, interfere the action of AchE in nerve and may lead to a severe impairment of nerve functions or even death. Therefore, insect AchE is the biological target of predominant insecticides used in agriculture. Biosensors are sensitive and can be used as dispoisable sensors for environmental control. In recent years, the use of AchEs in biosensor technology has gained enormous attention, in particular with respect to insecticide detection. The principle of biosensors using AchE as a biological recognition element is based on the inhibition the catalytic activity by the agents to be detected. We here present a strip-type biosensor based on AchE inhibition. In this study, acetylcholinesterase and PVA-SbQ (polyvinyl alcohol functionalized with methyl pyridinium methyl sulfate) were co-immobilized on immobilone-P membranes. Immobilization of the enzymes showed a stability in 6 months without activity loss in 4oC storage. Enzymes immobilized on surfaces of membrane responded to organophosphates and carbamate more sensitivitive than enzyme in solution. Organophosphates and carbamates concentrations could be detected by entrapped and surface immobilized enzymes, in 5 min. For chlorpyrifos, carbofuran, cabaryl, and methidathion, the detection limits of AChEstrip were similar to that of HPLC/GC method.
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KEYWORD
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acetylcholinesterase, PVA-SbQ, Organophosphates, rapid detection
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